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sting pathway sampler kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc sting pathway sampler kit
    FTZ@Fu SANs induce immunogenic cell death and potentiate immune checkpoint blockage therapy. ( A ) CLSM images of CRT expression in MDA-MB-231 cells after being treated with FZ, FT, FTZ, and FTZ@Fu MNCs (5 µg/ml) for 24 h. Bar = 100 μm. ( B ) Extracellular ATP release by MDA-MB-231 cells treated with FTZ@Fu (0–20 µg/ml) (left panel) or different MNC formulations (right panel). ( C ) CLSM images of γH2AX expression in MDA-MB-231 cells after being treated with MNCs (5 µg/ml) for 24 h. Bar =100 μm. Quantification of γH2AX-positive cells is shown in the right panel. ( D ) Western blot showing activation of the <t>sGAS/STING</t> <t>pathway</t> in MDA-MB-231 cells treated with MNCs. ( E ) Flow cytometric analysis of dendritic cell maturation (left panel), and quantitative data are shown in the right panel. ( F ) Quantification of dendritic cell-mediated tumor cell phagocytosis by a flow cytometric analysis
    Sting Pathway Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+reactive+sting+pathway+antibody+sampler+kit/Human-Reactive+STING+Pathway+Antibody+Sampler+Kit/pmc12128240-388-29-34
    Average 93 stars, based on 16 article reviews
    sting pathway sampler kit - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy"

    Article Title: Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-025-03473-0

    FTZ@Fu SANs induce immunogenic cell death and potentiate immune checkpoint blockage therapy. ( A ) CLSM images of CRT expression in MDA-MB-231 cells after being treated with FZ, FT, FTZ, and FTZ@Fu MNCs (5 µg/ml) for 24 h. Bar = 100 μm. ( B ) Extracellular ATP release by MDA-MB-231 cells treated with FTZ@Fu (0–20 µg/ml) (left panel) or different MNC formulations (right panel). ( C ) CLSM images of γH2AX expression in MDA-MB-231 cells after being treated with MNCs (5 µg/ml) for 24 h. Bar =100 μm. Quantification of γH2AX-positive cells is shown in the right panel. ( D ) Western blot showing activation of the sGAS/STING pathway in MDA-MB-231 cells treated with MNCs. ( E ) Flow cytometric analysis of dendritic cell maturation (left panel), and quantitative data are shown in the right panel. ( F ) Quantification of dendritic cell-mediated tumor cell phagocytosis by a flow cytometric analysis
    Figure Legend Snippet: FTZ@Fu SANs induce immunogenic cell death and potentiate immune checkpoint blockage therapy. ( A ) CLSM images of CRT expression in MDA-MB-231 cells after being treated with FZ, FT, FTZ, and FTZ@Fu MNCs (5 µg/ml) for 24 h. Bar = 100 μm. ( B ) Extracellular ATP release by MDA-MB-231 cells treated with FTZ@Fu (0–20 µg/ml) (left panel) or different MNC formulations (right panel). ( C ) CLSM images of γH2AX expression in MDA-MB-231 cells after being treated with MNCs (5 µg/ml) for 24 h. Bar =100 μm. Quantification of γH2AX-positive cells is shown in the right panel. ( D ) Western blot showing activation of the sGAS/STING pathway in MDA-MB-231 cells treated with MNCs. ( E ) Flow cytometric analysis of dendritic cell maturation (left panel), and quantitative data are shown in the right panel. ( F ) Quantification of dendritic cell-mediated tumor cell phagocytosis by a flow cytometric analysis

    Techniques Used: Expressing, Western Blot, Activation Assay

    Related Articles

    other:

    Article Title: Activation of cGAS-STING Signal to Inhibit the Proliferation of Bladder Cancer: The Immune Effect of Cisplatin.
    Article Snippet: Specific information of primary antibodies: Human-Reactive STING Pathway Antibody Sampler Kit (1:1000; cat no. #38866;CST, Danvers, MA, USA), MouseReactive STING Pathway Antibody Sampler Kit (1:1000; cat no. #16029; CST, Danvers, MA, USA), γ-H2A.X (1:1000; cat no. ab81299; Abcam, Cambridge, England), Rad51(1:1000; cat no. ab133534; Abcam), GAPDH (1:5000; cat no. ab8245; Abcam), and Histone H3 (1:1000; cat no. ab1791; Abcam).

    Article Title: Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers.
    Article Snippet: Human-Reactive STING Pathway Antibody Sampler Kit (38,866, CST), Mouse-Reactive STING Pathway Antibody Sampler Kit (16,029, CST), and α-GAPDH (5174, CST) were used in this assay.

    Article Title: Cisplatin Inhibits Bladder Cancer Proliferation Through cGAS-STING Pathway.
    Article Snippet: The information of abovementioned primary antibodies: Human-Reactive STING Pathway Antibody Sampler Kit (1:1, 000; cat no. #38866; Cell signaling technology, CST), Mouse-Reactive STING Pathway Antibody Sampler Kit (1:1, 000; cat no. #16029; CST), γ-H2A.X (1:1, 000; cat no. ab81299; Abcam), Rad51(1:1, 000; cat no. ab133534; Abcam), GAPDH (1:5, 000; cat no. ab8245; Abcam) and Histone H3 (1:1, 000; cat no. ab1791; Abcam).

    Article Title: α-Mangostin inhibits LPS-induced bone resorption by restricting osteoclastogenesis via NF-κB and MAPK signaling
    Article Snippet: The specific primary antibodies obtained from Cell Signaling Technology (Danvers, MA, USA) were as follows: c-Fos (#2250), NFATc1 (#8032), β-tubulin (#2146), phospho-JNK (#4668), IκBα (#4814), phospho-IκBα (#2859), p65 (#8242), phospho-p65 (#3033), phospho-IκB kinase (IKK)α/β (#2697), IKKβ (#8943), extracellular signal-related kinase (ERK) (#4695), phospho-ERK (#4370), JNK 1/2 (#9252), p38 (#9212), phospho-p38 (#4511), tartrate-resistant acid phosphatase (TRAP) (#92345), Human-reactive STING pathway antibody sampler kit (#38866).

    Article Title: Activation of cGAS-STING Signal to Inhibit the Proliferation of Bladder Cancer: The Immune Effect of Cisplatin
    Article Snippet: Specific information of primary antibodies: Human-Reactive STING Pathway Antibody Sampler Kit (1:1000; cat no. #38866;CST, Danvers, MA, USA), Mouse-Reactive STING Pathway Antibody Sampler Kit (1:1000; cat no. #16029; CST, Danvers, MA, USA), γ-H2A.X (1:1000; cat no. ab81299; Abcam, Cambridge, England), Rad51(1:1000; cat no. ab133534; Abcam), GAPDH (1:5000; cat no. ab8245; Abcam), and Histone H3 (1:1000; cat no. ab1791; Abcam).

    Article Title: Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers
    Article Snippet: Human-Reactive STING Pathway Antibody Sampler Kit (38,866, CST), Mouse-Reactive STING Pathway Antibody Sampler Kit (16,029, CST), and α-GAPDH (5174, CST) were used in this assay.

    Western Blot:

    Article Title: Nuclear translocation of ISG15 regulated by PPP2R2B inhibits cisplatin resistance of bladder cancer
    Article Snippet: .. Antibodies used for western blotting were as follows: PPP2R2B (ab157461, Abcam), GAPDH (60004-1-Ig, Proteintech), beta tubulin (10068-1-AP, Proteintech), phospho-DNA PKcs (Ser2056) (ab124918, Abcam), phospho-ATR (Ser428) (2853, CST), phospho-ATM (Ser1981) (5883, CST), ISG15 (15981-1-AP, Proteintech), anti-phosphoserine/threonine (PP2551, ECMbio), IPO5 (sc-55,527, Santa Cruz), LaminA/C (10298-1-AP, Proteintech), Human-Reactive STING Pathway Antibody Sampler Kit (38,866, CST), FLAG (F1804, Sigma), HA (51064-2-AP, Proteintech), STAT1 (9172, CST), pSTAT1 (9167, CST), H3 (17168-1-AP, Proteintech), H3K9me3 (ab8898, Abcam), and SUV39H1 (05-615, Sigma). .. Cycloheximide (GC17198) was from GLPBIO (America); Cisplatin (HY-17,394), LB-100 (HY-18,597) and chaetocin (HY-N2019) were from MedChemExpress (China).

    Article Title: Nuclear translocation of ISG15 regulated by PPP2R2B inhibits cisplatin resistance of bladder cancer.
    Article Snippet: .. Antibodies used for western blotting were as follows: PPP2R2B (ab157461, Abcam), GAPDH (60004-1-Ig, Proteintech), beta tubulin (10068-1-AP, Proteintech), phosphoDNA PKcs (Ser2056) (ab124918, Abcam), phospho-ATR (Ser428) (2853, CST), phospho-ATM (Ser1981) (5883, CST), ISG15 (15981-1-AP, Proteintech), anti-phosphoserine/threonine (PP2551, ECMbio), IPO5 (sc-55,527, Santa Cruz), LaminA/C (10298-1-AP, Proteintech), Human-Reactive STING Pathway Antibody Sampler Kit (38,866, CST), FLAG (F1804, Sigma), HA (51064-2-AP, Proteintech), STAT1 (9172, CST), pSTAT1 (9167, CST), H3 (17168- 1-AP, Proteintech), H3K9me3 (ab8898, Abcam), and SUV39H1 (05-615, Sigma). .. Cycloheximide (GC17198) was from GLPBIO (America); Cisplatin (HY-17,394), LB-100 (HY-18,597) and chaetocin (HY-N2019) were from MedChemExpress (China).



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    Image Search Results


    FTZ@Fu SANs induce immunogenic cell death and potentiate immune checkpoint blockage therapy. ( A ) CLSM images of CRT expression in MDA-MB-231 cells after being treated with FZ, FT, FTZ, and FTZ@Fu MNCs (5 µg/ml) for 24 h. Bar = 100 μm. ( B ) Extracellular ATP release by MDA-MB-231 cells treated with FTZ@Fu (0–20 µg/ml) (left panel) or different MNC formulations (right panel). ( C ) CLSM images of γH2AX expression in MDA-MB-231 cells after being treated with MNCs (5 µg/ml) for 24 h. Bar =100 μm. Quantification of γH2AX-positive cells is shown in the right panel. ( D ) Western blot showing activation of the sGAS/STING pathway in MDA-MB-231 cells treated with MNCs. ( E ) Flow cytometric analysis of dendritic cell maturation (left panel), and quantitative data are shown in the right panel. ( F ) Quantification of dendritic cell-mediated tumor cell phagocytosis by a flow cytometric analysis

    Journal: Journal of Nanobiotechnology

    Article Title: Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy

    doi: 10.1186/s12951-025-03473-0

    Figure Lengend Snippet: FTZ@Fu SANs induce immunogenic cell death and potentiate immune checkpoint blockage therapy. ( A ) CLSM images of CRT expression in MDA-MB-231 cells after being treated with FZ, FT, FTZ, and FTZ@Fu MNCs (5 µg/ml) for 24 h. Bar = 100 μm. ( B ) Extracellular ATP release by MDA-MB-231 cells treated with FTZ@Fu (0–20 µg/ml) (left panel) or different MNC formulations (right panel). ( C ) CLSM images of γH2AX expression in MDA-MB-231 cells after being treated with MNCs (5 µg/ml) for 24 h. Bar =100 μm. Quantification of γH2AX-positive cells is shown in the right panel. ( D ) Western blot showing activation of the sGAS/STING pathway in MDA-MB-231 cells treated with MNCs. ( E ) Flow cytometric analysis of dendritic cell maturation (left panel), and quantitative data are shown in the right panel. ( F ) Quantification of dendritic cell-mediated tumor cell phagocytosis by a flow cytometric analysis

    Article Snippet: Specific primary antibodies are as following: GCLC (ab53179, Abcam), GPX4 (GTX54095, GeneTex), NCOA4 (#66849, Cell Signaling Technology), LC3B (#83506, Cell Signaling Technology), FTH1 (#4393, Cell Signaling Technology), and a STING pathway sampler kit (#38866, Cell Signaling Technology).

    Techniques: Expressing, Western Blot, Activation Assay